The figure below shows good transfer of protein standard bands on to the nitrocellulose membrane. Western blotting and detection technology pdf chapter ii. Use the protocol on page 22 to perform blotting of gels using the blotting roller. The iblot 2 dry blotting system is based on the dry blotting concept, utilizing a unique gel matrix technology. This format eliminat es the need for premade buffers or soaked filter paper, and minimiz es. The iblot 2 transfer stack conssits of a bottom stack and a top stack. Invitrogen catalog first page, datasheet, datasheet search, data sheet, datasheets, datasheet search site for electronic components and semiconductors, integrated circuits, diodes, triacs, semiconductors. This manual is supplied with the iblot 2 gel transfer device cat. Find available technical resources for the iblot dry blotting system including firmware updates, application notes and. Instructions for using the iblot 2 gel transfer device to perform dry blotting of proteins. Protein transfer technical handbook thermo fisher scientific.
Cast gels, visit our web site at or contact technical service see page 66. If we could afford to use precast gels and iblot and western detection kit, then it would be a good system for getting everything done in one day. Page 2 life technologies corporation 5781 van allen way carlsbad, ca 92008 for descriptions of symbols on product labels or product. Choosing a gel for your application to obtain the best results for your application, it is important to choose the. Suggestions are listed in the manual for transferring low and high molecular weight proteins. Features accuracy eliminate the subjectivity and usertouser ariability of manual cell counting. The gelfree, blotfree, handsfree simplewesternishere. All steps of the iblot western detection protocol are performed after gel electrophoresis and transfer of the proteins to a. Product information product contents this manual is supplied with the iblot 2 gel transfer device cat. View and download invitrogen iblot 2 user manual online. Rat liver extract was focused on biorads readystrip 11 cm ph 58 ipg strips and separated on anykd criterion tgx gels. The kits are available with antimouse or antirabbit. Do not trim the membrane or iblot gel transfer stacks to fit your gel size.
Place the cell culture dish on ice and wash the cells with icecold pbs. Invitrogen novex powerease 500 in stock, we buy sell. User manual corporate headquarters invitrogen corporation 1600 faraday avenue carlsbad, ca 92008 t. User manual iblot dry blotting system for dry, electroblotting of proteins from mini, midi, and epage gels, electroblotting of nucleic acids from agarose gels, and tbe polyacrylamide gels, and performing western detection on nitrocellulose or pvdf membranes rev. Instructions are provided below for semiwet blotting of nupage gels using the xcell ii blot module. Used to transfer proteins with the iblot gel transfer device. Invitrogen iblot transfer stack, nitrocellulose, mini. Invitrogen iblot transfer stack, nitrocellulose, mini iblot transfer stacks are used to transfer proteins 1428. This invitrogen novex powerease 500 power supply is used and in excellent condition.
Refer to the manual if you are using the iblot gel transfer device with the debubbling roller. For detailed instructions refer to the manual at iblot2. The beta star unit is of gmp design, meeting the intent of the fda gmps 21 crf, part 210, 211 and aami guidelines iso 1141993. Types of products this manual is supplied with the iblot gel transfer device cat.
Read unpacking instructions in the manual for set up instructions when using the iblot 2 gel transfer device for the first time. Invitrogen iblot dna transfer stack dna transfer stack. Invitrogen iblot ib1001 gel transfer device new 2794a. This unit was made by novex which was incorporated into invitrogen. From the innovative iblot dry blotting system that enables 7minute protein transfers to quality precut. Ge sievers 900 total organic carbon toc analyzer integrity tester. Invitrogen iblot transfer stack, pvdf, mini mini, 10 sets. Nupage technical guide thermo fisher scientific mafiadoc. Invitrogen iblot transfer stack, pvdf, regular size regular. Invitrogen iblot 2 dry blot system used electrophoresis. The iblot system is described as a dry system no buffer preparation required for 78 minute transfers of protein from page gels to nitrocellulose or pvdf membranes.
As it is, they are just too expensive, and i dont like the hot transfer, it will. Invitrogen sdspage and western blotting equipment iblot gel transfer device, benchpro 4100 toc analyzer. Use the blotting roller to remove any bubbles between layers of the stack. A549 whole cell lysates were prepared for sdspage and loaded onto a nupage 412% bistris gel 1. Palltronic flowstar iv filter integrity test instrument. Transfer efficiency of the thermo scientific pierce fast transfer system is comparable to existing methods. After electrophoresis, the gel was subjected to blotting using the iblot gel transfer device and iblot gel transfer stacks, mini with blotting roller as described in this manual. Transblot turbo transfer system life science education. Precast gels are capable of resolving proteins in the range of 2500 kda and nucleic acids in the range of 103000 bp. May 18, 2009 invitrogen iblot may182009 has anyone ever used invitrogen s iblot dry blotting system for western protein transfer. Jun 17, 2011 invitrogen offers one the most comprehensive portfolio of products for western analysis. For western detection the iblot western detection stack is assembled with your. Description quantity price single component reagents 10538810 aqualine complete 1 water equivalent 1mg h 2 oml 1l 111. For western detection the iblot western detection school university of texas.
Each iblot dna transfer stack contains sponges, cathode and anode electrodes and a nylon membrane, preassembled and primed with a proprietary formulation for optimum transfer of dna from the gel to the membrane. Dry blotting system iblot 2 laboratory equipment pdf manual download. The iblot 2 dry blotting system consists of the iblot 2 gel transfer device and associated iblot 2 transfer stacks sold separately. For western detection the iblot western detection stack is. To download the pdf, see application notes tab on this page. For more information on the xcell ii blot module, refer to the manual im9051 available at or contact technical support see page 56.
Higher sensitivity and better transfer efficiency is seen with the transblot turbo system in comparison to other blotting techniques. Western blotting using iblot 7minute blotting system. A selfcontained unit, the iblot device uses disposable blotting stacks with integrated nitrocellulose or pvdf membranes, offering the convenience of a. According to the manual, large proteins dont transfer well. The contents of the iblot 2 gel transfer device are listed below. The iblot transfer stacks are used to transfer proteins using the iblot gel transfer device. Using the iblot gel transfer device with blotting roller instructions are provided below to assemble the iblot gel transfer stack to perform blotting of mini, midi, or other gels using the iblot gel transfer device. While this is technically correct, the manual recommends an equilibration step of the gel in transfer buffer prior to iblot transfer for more complete transfer of mid to. Be certain to align the black with black and clear side with the red side of the transfer apparatus. Choose simple brightfield counting with the invitrogen countess ii automated cell counter, or the flexibility and upgradability of fluorescence detection with the invitrogen countess ii fl automated cell counter. From the innovative iblot dry blotting system that enables. Nov 03, 2010 the iblot western detection kits work with the iblot dry blotting system, enabling complete western detection in less than 30 minutes.
New, old stock invitrogen iblot gel transfer system manufacturer. Now with a new touchscreen, less consumable waste, and sturdy design, fast western transfer never looked better. Traditionally, this procedure consists of multiple steps, requires skilled labor, and takes up to two days to. The iblot 7minute blotting system efficiently and reliably blots proteins from polyacrylamide gels in 7 minutes or less without the need for additional buffers or an external power supply. Invitrogen iblot transfer stack, nitrocellulose, mini iblot transfer stacks are used to transfer proteins 1150. I have one, and it sounds great protein transfer in 7 minutes, but it almost sounds too good to be true. This data set demonstrates the successful transfer of the 1. The iblot 2 system is compatible with both polyvinylidene difluoride pvdf and nitrocellulose membranes, and has comparable performance to traditional wet transfer methods in a fraction of the time. General guidelines remove air bubbles as indicated in the protocol using the blotting roller. Invitrogen iblot transfer stack, pvdf, regular size. Refer to the manual for the iblot dry blotting system 250949 for details. The iblot 2 gel transfer device has a unique design, which, in conjunction with the patented gel matrix technology of the iblot 2 transfer stacks, results in a shortened distance between electrodes. Download operating manual of zygo newview 7300 laboratory equipment for free or view it online on.
Close the sandwich holder and place assembly into the gel box. Tps bench and mini img instruction manual agilent technologies was added on 201401 has been download 55 which last down load at 20141031 16. The nano glo hibit blotting systema,b,c simply and sensitively detects hibittagged proteins on nitrocellulose or pvdf membranes following sdspage and transfer figure 1. This manual is supplied with the iblot gel transfer device cat. The iblot 2 transfer stack consists of a bottom stack and a top stack sandwiching a prerun gel and a nitrocellulose 0. View online or download invitrogen iblot 2 user manual. Instructions for using the iblot gel transfer device to perform dry blotting of. The gel matrix of the bottom and top stack incorporate the appropriate anode and cathode buffers to act as ion reservoirs. General western blot protocol sample lysis preparation of lysate from cell culture 1. Transblot turbo transfer system biorad laboratories.
801 1565 1506 393 1318 1489 305 807 875 417 98 1095 1265 1431 507 1303 1472 1574 239 623 97 1520 545 616 315 73 233 1117 684 1275 181 130 324 22 490 246